Journal: iScience

Article Title: Sulfonylurea receptor 1 deficiency alleviates acute lung injury by activating PARP1 in epithelial cells

doi: 10.1016/j.isci.2026.117627

Figure Lengend Snippet: Glibenclamide disrupted SUR1-PARP1 interaction to restore DNA repair and attenuate apoptosis and inflammation in HBE cells (A) Schematic diagram of HBE cells with different treatments, followed by RNA sequencing analysis. Groups: control, 20 ng/mL; TNF-α, 100 μmol/L; glibenclamide plus TNF-α. n = 2 per group. (B) Venn diagram of differentially expressed genes. (C) GO and KEGG enrichment analyses of 280 genes in the intersection shown in (B). (D) RT-qPCR analysis of HBE cells with different treatments as indicated. (E) Co-immunoprecipitation assay in HBE cells transfected with FLAG-SUR1 plasmids and treated with 20 ng/mL TNF-α or 100 μmol/L glibenclamide as indicated. L.E., long exposure. (F) HBE cells were treated as in (E), then subjected to cytoplasmic and nuclear protein fractionation followed by western blotting. Cyto, cytoplasm; Nu, nucleus. (G) Western blot analysis of HBE cells pretreated with 100 μmol/L glibenclamide for 30 min followed by TNF-α or LPS co-treatment for 1 h. (H–J) ROS production, caspase-3 activity, and apoptosis in HBE cells under indicated treatments. (K–M) HBE cells were pretreated with 100 μmol/L glibenclamide, 5 μmol/L olaparib, or their combination for 24 h, followed by 20 ng/mL TNF-α stimulation, and subjected to western blotting, caspase-3 activity assay, and apoptosis analysis by flow cytometry. Gli, glibenclamide. Data are presented as mean ± SD ( n = 3). ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001. GO and KEGG enrichment analyses in (C) were performed using a hypergeometric test with false discovery rate (FDR) correction. Data in (D) and (G–M) were analyzed using one-way ANOVA followed by Tukey’s multiple-comparisons test. Representative images are shown.

Article Snippet: Caspase-3 activity to show cell apoptosis was determined using the Caspase 3 Activity Assay Kit (C1115; Beyotime Biotechnology) following the manufacturer’s protocol.

Techniques: RNA Sequencing, Analysis, Control, Quantitative RT-PCR, Co-Immunoprecipitation Assay, Transfection, Fractionation, Western Blot, Activity Assay, Caspase-3 Activity Assay, Flow Cytometry

Journal: iScience

Article Title: Sulfonylurea receptor 1 deficiency alleviates acute lung injury by activating PARP1 in epithelial cells

doi: 10.1016/j.isci.2026.117627

Figure Lengend Snippet:

Article Snippet: Caspase-3 activity to show cell apoptosis was determined using the Caspase 3 Activity Assay Kit (C1115; Beyotime Biotechnology) following the manufacturer’s protocol.

Techniques: Cell-Signaling, Recombinant, Transfection, Caspase-3 Activity Assay, Protein Extraction, Knock-Out, Sequencing, Control, Plasmid Preparation, Software, Analysis

Journal: Molecular Biology Reports

Article Title: miR-486 attenuates myocardial ischaemia/reperfusion injury by inhibiting apoptosis and autophagy activation via the FOXO1/PTEN/AKT/mTOR axis

doi: 10.1007/s11033-026-12913-5

Figure Lengend Snippet: MiR-486 inhibits cardiomyocyte apoptosis in vitro and in vivo. ( A ) Representative western blot images and quantitative analysis of the Bax/Bcl-2 ratio and cleaved caspase-3/caspase-3 ratio in H9c2 cells under control conditions or after OGD/R treatment. ( B ) Representative Western blot images and quantitative analysis of the Bax/Bcl-2 ratio and cleaved caspase-3/caspase-3 ratio in H9c2 cells transfected with miR-486 mimic or mimic NC under OGD/R conditions. ( C ) Representative western blot images and quantitative analysis of the Bax/Bcl-2 ratio and cleaved caspase-3/caspase-3 ratio in H9c2 cells transfected with miR-486 inhibitor or inhibitor NC under OGD/R conditions. (D ) Representative western blot images and quantitative analysis of the Bax/Bcl-2 ratio and cleaved caspase-3/caspase-3 ratio in rat myocardial tissues from the indicated groups. Data are presented as mean ± SD. For in vivo experiments, n = 5 per group. For in vitro experiments, data were obtained from three independent experiments ( n = 3). Statistical significance was determined using one-way ANOVA followed by Tukey’s multiple comparisons test. Statistical comparisons between the indicated groups are directly shown in the graphs.* P < 0.05, ** P < 0.01, *** P < 0.001,**** P < 0.0001. AAV, adeno-associated virus; NC, negative control; OGD/R, oxygen-glucose deprivation/reperfusion

Article Snippet: Cleaved-Caspase 3 , Cell Signalling Technology , 9662 , 17, 19.

Techniques: In Vitro, In Vivo, Western Blot, Analysis, Control, Transfection, Virus, Negative Control

Journal: Molecular Biology Reports

Article Title: miR-486 attenuates myocardial ischaemia/reperfusion injury by inhibiting apoptosis and autophagy activation via the FOXO1/PTEN/AKT/mTOR axis

doi: 10.1007/s11033-026-12913-5

Figure Lengend Snippet: FOXO1 and PTEN mediate the regulatory effects of miR-486 on OGD/R-induced apoptosis and autophagy activation in H9c2 cells. ( A ) Representative Western blot images and quantitative analysis of the Bax/Bcl-2 ratio, cleaved caspase-3/caspase-3 ratio, Beclin1, LC3-II/GAPDH, and p62 expression in H9c2 cells co-transfected with si-FOXO1 or siRNA NC and miR-486 inhibitor or inhibitor NC under OGD/R conditions. (B) Representative western blot images and quantitative analysis of the Bax/Bcl-2 ratio, cleaved caspase-3/caspase-3 ratio, Beclin1, LC3-II/GAPDH, and p62 expression in H9c2 cells co-transfected with si-PTEN or siRNA NC and miR-486 inhibitor or inhibitor NC under OGD/R conditions. Data are presented as mean ± SD from three independent experiments ( n = 3). Statistical significance was determined using one-way ANOVA followed by Tukey’s multiple comparisons test. Statistical comparisons between the indicated groups are directly shown in the graphs.* P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001. NC negative control; OGD/R, oxygen-glucose deprivation/reperfusion

Article Snippet: Cleaved-Caspase 3 , Cell Signalling Technology , 9662 , 17, 19.

Techniques: Activation Assay, Western Blot, Analysis, Expressing, Transfection, Negative Control

Journal: Molecular Biology Reports

Article Title: miR-486 attenuates myocardial ischaemia/reperfusion injury by inhibiting apoptosis and autophagy activation via the FOXO1/PTEN/AKT/mTOR axis

doi: 10.1007/s11033-026-12913-5

Figure Lengend Snippet: Akt and mTOR are involved in mediating the effects of miR-486 on OGD/R-induced apoptosis and autophagy activation in H9c2 cells. ( A ) Representative western blot images and quantitative analysis of the Bax/Bcl-2 ratio, cleaved caspase-3/caspase-3 ratio, Beclin1, LC3-II/GAPDH, and p62 expression in H9c2 cells treated with MK2206 or vehicle and transfected with miR-486 mimic or mimic NC under OGD/R conditions. ( B ) Representative western blot images and quantitative analysis of the Bax/Bcl-2 ratio, cleaved caspase-3/caspase-3 ratio, Beclin1, LC3-II/GAPDH, and p62 expression in H9c2 cells treated with rapamycin or vehicle and transfected with miR-486 mimic or mimic NC under OGD/R conditions. Data are presented as mean ± SD from three independent experiments ( n = 3). Statistical significance was determined using one-way ANOVA followed by Tukey’s multiple comparisons test. Statistical comparisons between the indicated groups are directly shown in the graphs.* P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001. NC negative control; OGD/R, oxygen-glucose deprivation/reperfusion

Article Snippet: Cleaved-Caspase 3 , Cell Signalling Technology , 9662 , 17, 19.

Techniques: Activation Assay, Western Blot, Analysis, Expressing, Transfection, Negative Control